RECORD
Assessment of Forest Biomass Management Practices through Fungal Community Sampling: Wood-Inhabiting Fruiting Body Surveys and DNA-Based Analyses of Wood Stakes in a Western Conifer Forest of North America
- Title:
- Assessment of Forest Biomass Management Practices through Fungal Community Sampling: Wood-Inhabiting Fruiting Body Surveys and DNA-Based Analyses of Wood Stakes in a Western Conifer Forest of North America
- Creator:
- Draeger, Kymberly R.
- Date Created:
- 2018
- Description:
- To assess the effects of biomass harvesting treatments and compensatory soil amendments on wood-inhabiting fungal communities, two methods of fungal sampling (collection of fruiting bodies and DNA-based methods) were used to analyze changes in wood-inhabiting fungal communities in a conifer-dominated forest in western North America. Plots were established with biomass retention at four levels and with four soil amendment treatments. Fruiting body surveys of woody substrates yielded 1,002 observations over 3 years, comprising 129 mostly basidiomycete species. Community composition of fungal fruiting bodies was significantly influenced by biomass treatments in 2014 (p = 0.003), while there was no significant effect of soil amendment treatments relative to community composition. DNA-based methods, analyzing wood stakes on or within soil, revealed 2,316 different operational taxonomic units (OTUs) of mostly ascomycete species. Fungal community composition within wood stakes as indicated by high-throughput amplicon sequencing was influenced by biomass treatments all 3 years (p < 0.04), while soil amendments were found to significantly influence fungal community composition in 2015 and 2017 (p < 0.04). The two sampling methods revealed different fungal communities. Fruiting body surveys sampled a range of wood substrates and identified important forest pathogens. DNA-based methods produced more data, identified more OTUs (p < 0.001) and were more sensitive to treatments, especially soil amendment treatments. Both methods had advantages and disadvantages, and success with each method depends on the study, the questions being asked, and the researcher’s skills. The potential for biomass harvesting and soil amendments to alter fungal communities in conifer-dominated forests of western North America has implications for forest health and productivity. Within this limited study, removal of thinned woody biomass did not negatively influence fungal community composition.
- Document Type:
- Dissertation
- Subjects:
- UIEF biomass harvesting forest biomass fungal community sampling biomass retention soil amendment treatments forest health forest productivity forest pathology
- Location:
- UIEF
- Latitude:
- 46.869607
- Longitude:
- -116.733856
- Publisher:
- ProQuest Dissertations Publishing
- Department:
- Forest, Rangeland, and Fire Sciences
- Type:
- Text
Source
- Preferred Citation:
- "Assessment of Forest Biomass Management Practices through Fungal Community Sampling: Wood-Inhabiting Fruiting Body Surveys and DNA-Based Analyses of Wood Stakes in a Western Conifer Forest of North America", UIEF Research Exchange, University of Idaho Library Digital Collections, https://www.lib.uidaho.edu/digital/uief/items/uief_0155.html
Rights
- Rights:
- In copyright, educational use permitted.
- Standardized Rights:
- http://rightsstatements.org/vocab/InC-EDU/1.0/