RECORD
Exploring Softwoods Fiber Formation Process in Douglas-fir (Pseudotsuga menziesii) In-Vitro Model System Using Proteomics Approach
- Title:
- Exploring Softwoods Fiber Formation Process in Douglas-fir (Pseudotsuga menziesii) In-Vitro Model System Using Proteomics Approach
- Creator:
- Laniak-Dziedzic, Jowita A.
- Date Created:
- 2012-08
- Description:
- Preudotsuga menziesii (Douglas-fir) is one of the most economically important timber species in Pacific Northwest. This demand has put a pressure on the wood industry to develop more sustainable strategies to improve yields and wood quality. One possible way to address this goal is a genetic modification of Douglas-fir creating faster to grow, pathogen resistant and most importantly with improved wood quality tree line. To achieve such an aim is to first characterize all involved molecular pathways and future modification targets. This can be especially difficult in case of a non-modal organism such as Douglas-fir. Without organism genome sequenced, another approach used in this tape of studies in a direct investigation of involved proteins my means of mass spectrometry. Comparative analysis of proteome of wood fibers, at different developmental stages, can be used to get an insight into the mechanism behind wood formation. Methods: In order to study wood fiber formation process in Douglas-fir an in-vitro model system was employed. In this approach, differentiation processes were monitored by tracking wood fiber cell wall development (primary to secondary cell wall) using polarized microscopy imaging. To analyze proteins expressed at various stages of in-vitro tracheids formation several methods of protein extraction, purification and separation were investigated and a phenol-based approach was selected for subsequent studies. Protein expressed in various samples (in-vitro and in-vivo collected specimens) were then identified using a proteomics approach utilizing reverse-phase liquid chromatography coupled to tandem mass spectrometry
- Document Type:
- Dissertation
- Subjects:
- UIEF Douglas-fir pseudotsuga menziesii softwood wood fiber forest products genetic modification harvest sustainability wood fiber formation forest ecology
- Location:
- UIEF
- Latitude:
- 46.869607
- Longitude:
- -116.733856
- Publisher:
- ProQuest Dissertations Publishing
- Department:
- Forest, Rangeland, and Fire Sciences
- Type:
- Text
Source
- Preferred Citation:
- "Exploring Softwoods Fiber Formation Process in Douglas-fir (Pseudotsuga menziesii) In-Vitro Model System Using Proteomics Approach", UIEF Research Exchange, University of Idaho Library Digital Collections, https://www.lib.uidaho.edu/digital/uief/items/uief_0202.html
Rights
- Rights:
- In copyright, educational use permitted.
- Standardized Rights:
- http://rightsstatements.org/vocab/InC-EDU/1.0/