ETD RECORD
Effects of the carbohydrate level and of dietary protein source on growth performance, plasma parameters and hepatic gene expression in rainbow trout
Citation
Wacyk, Jurij M.. (2009). Effects of the carbohydrate level and of dietary protein source on growth performance, plasma parameters and hepatic gene expression in rainbow trout. Theses and Dissertations Collection, University of Idaho Library Digital Collections. https://www.lib.uidaho.edu/digital/etd/items/etd_13.html
- Title:
- Effects of the carbohydrate level and of dietary protein source on growth performance, plasma parameters and hepatic gene expression in rainbow trout
- Author:
- Wacyk, Jurij M.
- Date:
- 2009
- Keywords:
- Rainbow trout--Feeding and feeds
- Program:
- Animal Physiology
- Abstract:
- Two experiments were conducted to study specific aspects of fish meal replacement in rainbow trout diets. The first one studied the effects of increasing levels of digestible carbohydrates and showed that rainbow trout is able to maximize protein retention with 15% of CHO inclusion without compromising fish performance. This effect seems to be the result of metabolic adaptations involving changes in transaminase expression as well as in genes associated with REDOX environment in the liver. Higher levels of carbohydrate inclusion (>15%) seems to overcome the ability of the fish to adapt to the diets as reflected by higher levels of expression of REDOX genes, higher whole body fat deposits and lower protein retentions observed for this group of fish. The second experiment, used amino acid balanced diets to study the effects of soy protein isolate (SPI) as the sole protein source in diets supplemented with increasing levels of branched chain amino acids (BCAA). Fish performance was negatively affected by replacing fishmeal (FM) using SPI regardless of the level of BCAA supplementation. The fish receiving the SPI-diets also presented significantly higher levels of indispensable amino acids in plasma and lower levels of hepatic transaminase expression compared to fish fed FM-based diets. The former fish presented significantly higher levels of expression of the REDD-1 gene, which has been associated with a reduced ability to synthesize protein through the TOR cascade in mammals. Glucose-6-phosphate dehydrogenase was greatly affected by the dietary treatments, showing higher levels of expression in the liver of fish fed the SPI-diets. The BCAA supplementation showed an unexpected effect reducing whole body fat in this study reducing also the levels of lysine and threonine in the plasma of fish regardless of the protein source of the diets.
- Description:
- Thesis (Ph. D., Animal Physiology)--University of Idaho, June 2009.
- Major Professor:
- Ronald W. Hardy.
- Defense Date:
- June 2009.
- Type:
- Text
- Format Original:
- xix, 174 leaves :ill. ;29 cm.
- Format:
- record
Rights
- Rights:
- In Copyright - Educational Use Permitted. For more information, please contact University of Idaho Library Special Collections and Archives Department at libspec@uidaho.edu.
- Standardized Rights:
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